Why didn%27t you get isolated colonies from the pour plate if the broth culture was not diluted first

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  • Those colonies on plate B are probably not bacteria. If they are a very opaque white with a very sharp outline that sometimes makes a depression in the agar, they are most likely fungus. They will grow very slowly in overnight liquid culture or will form a stringy growth if you look closely.

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    But it is okay. It is very simply to pick small colonies. Here is one trick. Buy some toothpicks and place them in a beaker. Cover it with aluminium and send it for autoclaving. AFter that, you can always pick your tiny colonies with the toothpick and drink the toothpick into your culture tube containing LB broth and the appropriate antibiotics!

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    ⇒ Pour Plate culture technique is also used as a means of determining the numbers of viable organisms in a liquid such as water, milk, Urine, or Broth cultures as well as to determine the hemolytic activity of deep colonies of some bacteria, such as the Streptococci, by using an agar medium containing blood.

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    Cover less if you plan a four-way or five-way streak plate. Figure 2 (above) shows a pattern for four-way dilution streaking. 3. Repeat step 2 for one to three more sections, flaming and cooling the loop between sections. 4. Invert and incubate the plate and after the colonies have grown up inspect to see whether or not you have a pure culture.

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    The reason why you have to incubate your bacteria first in LB broth is for the bacteria to have time to express its antibiotic resistant gene present so when you plate it positive clones with the...

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    A: Since you have asked multiple questions, we will solve the first complete question for you. Q: transfer and isolation techniques:Why should the loop be flamed at least once during the streak plat... A: Streak plate technique is used for isolation of the pure culture of organisms from a mixed...

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We get colonies, but when picked and grown in liquid culture they either fail to grow or grow extremely poorly. This happens with multiple vectors for this one construct.
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